pampk α1  (Biorbyt)

Journal: Brain research bulletin

Article Title: Hindbrain Dorsal Vagal Complex AMPK Controls Hypothalamic Gluco-regulatory Transmitter and Counter-Regulatory Hormone Responses to Hypoglycemia

doi: 10.1016/j.brainresbull.2018.11.016

Figure Lengend Snippet: Effects of Caudal Fourth Ventricular (CV4) Administration of the 5’-adenosine monophosphate-activated protein kinase (AMPK) Inhibitor Compound C (Cc) on Hypoglycemic Patterns of Arcuate Hypothalamic Nucleus (ARH) AMPK Activation and Neuropeptide Y (NPY), and Proopiomelanocortin (POMC) Protein Expression. Groups of adult male rats were pretreated by vehicle (V) or Cc injection into the CV4 prior to sc insulin (INS) administration. Data depict mean normalized protein optical density (O.D) measures of ARH AMPK (Panel A), phosphoAMPK (pAMPK; Panel B), NPY (Panel C), and POMC (Panel D) + S.E.M. for the following treatment groups: V/V (solid white bars; n=5); V/INS (solid gray bars; n=5); Cc/INS (diagonal-striped gray bars; n=5). *p<0.05; **p<0.01; ***p<0.001.

Article Snippet: Proteins of interest were probed with primary polyclonal antisera raised in rabbit against AMPK α1/2 (1:2,000; 2532s; Cell Signaling Technology, Danvers, MA), pAMPK α1/2 (Thr 172; 1:2,000; 2535; Cell Signaling Technol.), RFamide related peptide-1 (RFRP-1; 1:1,000, sc-67010; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), or neuronal nitric oxide synthase (nNOS; 1:2,000, NBP1–39681; Novus Biologicals, LLC, Littleton, CA), or raised in goat against NPY (1:2,500, NBP1–46535; Novus Biol.), POMC (1:2,000, NB100–1533; Novus Biol.), GAD 65/67 (1:2,000, AB1511; EMD Millipore, Burlington, MA), RFamide related peptide-3 (RFRP-3; 1:1,000, sc-32380; Santa Cruz Biotechnol.), or ORX-A (1:1,000, sc-8070; Santa Cruz Biotechnol.).

Techniques: Activation Assay, Expressing, Injection

Journal: Brain research bulletin

Article Title: Hindbrain Dorsal Vagal Complex AMPK Controls Hypothalamic Gluco-regulatory Transmitter and Counter-Regulatory Hormone Responses to Hypoglycemia

doi: 10.1016/j.brainresbull.2018.11.016

Figure Lengend Snippet: Impact of Cc Pretreatment on Ventromedial Hypothalamus Nucleus (VMN) AMPK and, Glutamate Decarboxylate65/67 (GAD65/67), and Neuronal Nitric Oxide Synthase (nNOS) Protein Expression during Insulin-Induced Hypoglycemia (IIH). Results show mean normalized VMN AMPK (Panel A), pAMPK (Panel B), GAD65/67 (Panel C), and nNOS (Panel D) O.D. values + S.E.M. for V/V, V/INS, and Cc/INS treatment groups (n=5/group). *p<0.05; **p<0.01; ***p<0.001.

Article Snippet: Proteins of interest were probed with primary polyclonal antisera raised in rabbit against AMPK α1/2 (1:2,000; 2532s; Cell Signaling Technology, Danvers, MA), pAMPK α1/2 (Thr 172; 1:2,000; 2535; Cell Signaling Technol.), RFamide related peptide-1 (RFRP-1; 1:1,000, sc-67010; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), or neuronal nitric oxide synthase (nNOS; 1:2,000, NBP1–39681; Novus Biologicals, LLC, Littleton, CA), or raised in goat against NPY (1:2,500, NBP1–46535; Novus Biol.), POMC (1:2,000, NB100–1533; Novus Biol.), GAD 65/67 (1:2,000, AB1511; EMD Millipore, Burlington, MA), RFamide related peptide-3 (RFRP-3; 1:1,000, sc-32380; Santa Cruz Biotechnol.), or ORX-A (1:1,000, sc-8070; Santa Cruz Biotechnol.).

Techniques: Expressing

Journal: Brain research bulletin

Article Title: Hindbrain Dorsal Vagal Complex AMPK Controls Hypothalamic Gluco-regulatory Transmitter and Counter-Regulatory Hormone Responses to Hypoglycemia

doi: 10.1016/j.brainresbull.2018.11.016

Figure Lengend Snippet: Role of Dorsal Vagal Complex (DVC) AMPK in IIH-Associated Patterns of Dorsomedial Hypothalamus Nucleus (DMN) AMPK Activation and RFamide-Related Peptide-1 (RFRP-1) and −3 (RFRP-3) Protein Expression. Data illustrate indicate mean normalized DMN AMPK (Panel A), pAMPK (Panel B), RFRP-1 (Panel C), and RFRP-3 (Panel D) O.D. values + S.E.M. for groups of V-pretreated, V-injected (solid white bars; n=5) controls and INS-injected animals pretreated with V (solid gray bars; n=5) or Cc (diagonal-striped gray bars; n=5/group). *p<0.05; **p<0.01; ***p<0.001.

Article Snippet: Proteins of interest were probed with primary polyclonal antisera raised in rabbit against AMPK α1/2 (1:2,000; 2532s; Cell Signaling Technology, Danvers, MA), pAMPK α1/2 (Thr 172; 1:2,000; 2535; Cell Signaling Technol.), RFamide related peptide-1 (RFRP-1; 1:1,000, sc-67010; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), or neuronal nitric oxide synthase (nNOS; 1:2,000, NBP1–39681; Novus Biologicals, LLC, Littleton, CA), or raised in goat against NPY (1:2,500, NBP1–46535; Novus Biol.), POMC (1:2,000, NB100–1533; Novus Biol.), GAD 65/67 (1:2,000, AB1511; EMD Millipore, Burlington, MA), RFamide related peptide-3 (RFRP-3; 1:1,000, sc-32380; Santa Cruz Biotechnol.), or ORX-A (1:1,000, sc-8070; Santa Cruz Biotechnol.).

Techniques: Activation Assay, Expressing, Injection

Journal: Brain research bulletin

Article Title: Hindbrain Dorsal Vagal Complex AMPK Controls Hypothalamic Gluco-regulatory Transmitter and Counter-Regulatory Hormone Responses to Hypoglycemia

doi: 10.1016/j.brainresbull.2018.11.016

Figure Lengend Snippet: Effects of Intra-CV4 Cc Administration on Lateral Hypothalamic Area (LHA) AMPK Activity and Orexin-A (ORX-A) Activity and RFamide-Related Peptide-1 (RFRP-1) and −3 (RFRP-3) Protein Expression in Hypoglycemic Male Rats. Data indicate mean normalized LHA AMPK (Panel A), pAMPK (Panel B), and ORX-A (Panel C) O.D. values + S.E.M. for V/V, V/INS, and Cc/INS treatment groups (n=5/group). *p<0.05; **p<0.01; ***p<0.001.

Article Snippet: Proteins of interest were probed with primary polyclonal antisera raised in rabbit against AMPK α1/2 (1:2,000; 2532s; Cell Signaling Technology, Danvers, MA), pAMPK α1/2 (Thr 172; 1:2,000; 2535; Cell Signaling Technol.), RFamide related peptide-1 (RFRP-1; 1:1,000, sc-67010; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), or neuronal nitric oxide synthase (nNOS; 1:2,000, NBP1–39681; Novus Biologicals, LLC, Littleton, CA), or raised in goat against NPY (1:2,500, NBP1–46535; Novus Biol.), POMC (1:2,000, NB100–1533; Novus Biol.), GAD 65/67 (1:2,000, AB1511; EMD Millipore, Burlington, MA), RFamide related peptide-3 (RFRP-3; 1:1,000, sc-32380; Santa Cruz Biotechnol.), or ORX-A (1:1,000, sc-8070; Santa Cruz Biotechnol.).

Techniques: Activity Assay, Expressing

Journal: Brain research bulletin

Article Title: Hindbrain Dorsal Vagal Complex AMPK Controls Hypothalamic Gluco-regulatory Transmitter and Counter-Regulatory Hormone Responses to Hypoglycemia

doi: 10.1016/j.brainresbull.2018.11.016

Figure Lengend Snippet: A2 Noradrenergic Neuron AMPK, pAMPK, Dopamine-Beta-Hydroxylase (DβH), and Monocarboxylate Transporter-2 (MCT) Protein Responses to IIH; Impact of Cc Pretreatment. Data illustrate mean A2 AMPK (Panel A), pAMPK (Panel B), DβH (Panel C), and MCT (Panel D) protein O.D. measures + S.E.M. for V/V, V/INS, and Cc/INS treatment groups (n=5/group). *p<0.05; **p<0.01; ***p<0.001.

Article Snippet: Proteins of interest were probed with primary polyclonal antisera raised in rabbit against AMPK α1/2 (1:2,000; 2532s; Cell Signaling Technology, Danvers, MA), pAMPK α1/2 (Thr 172; 1:2,000; 2535; Cell Signaling Technol.), RFamide related peptide-1 (RFRP-1; 1:1,000, sc-67010; Santa Cruz Biotechnology, Inc., Santa Cruz, CA), or neuronal nitric oxide synthase (nNOS; 1:2,000, NBP1–39681; Novus Biologicals, LLC, Littleton, CA), or raised in goat against NPY (1:2,500, NBP1–46535; Novus Biol.), POMC (1:2,000, NB100–1533; Novus Biol.), GAD 65/67 (1:2,000, AB1511; EMD Millipore, Burlington, MA), RFamide related peptide-3 (RFRP-3; 1:1,000, sc-32380; Santa Cruz Biotechnol.), or ORX-A (1:1,000, sc-8070; Santa Cruz Biotechnol.).

Techniques: